Projektbeschreibung
Infektionskrankheiten stellen weltweit weiterhin eine große gesundheitliche und sozioökonomische Herausforderung dar, insbesondere in Ländern mit niedrigem und mittlerem Einkommen. Die Entwicklung kostengünstiger, sicherer und leicht skalierbarer Impfstoff-Plattformen ist daher von zentraler Bedeutung für die globale Gesundheitsvorsorge.
Ziel der vorliegenden Studie ist es, das HPV-16 L1-Antigen in Kopfsalat als essbarer Pflanze zu exprimieren, um eine alternative, kosteneffiziente Impfstoffstrategie zu etablieren. Dabei baut das Projekt auf erfolgreichen Vorarbeiten der Arbeitsgruppe auf, in denen bereits Antigene gegen Tuberkulose, Dengue-Virus und Salmonella erfolgreich pflanzenbasiert exprimiert und funktionell charakterisiert wurden. Untersucht werden die stabile Integration und Expression des Transgens sowie die korrekte Proteinexpression und Immunogenität des L1-Antigens. Abschließend soll das immunogene Potenzial des pflanzenbasierten Impfstoffkandidaten in präklinischen Mausmodellen evaluiert werden.
18 Monate: 2026-2027
Weitere Infos
Prof. Dr. Mohammad Tahir Waheed as collaborating equal partner and PI in Pakistan
Ijaz, F., Sameeullah, M., Farid, A., Malik, M. S., Batool, N., Mirza, B., Timko, M. P., Liu, H., Lössl, A. G., & Waheed, M. T. (2025). In silico designing and characterization of outer membrane protein (OmpC) gene from Salmonella enterica and its expression in Nicotiana tabacum for developing a plant-based vaccine against salmonellosis. Microbial Pathogenesis, 199, 107225. https://doi.org/10.1016/j.micpath.2024.107225
Latif S, Gottschamel J, Syed T, Younus I, Gull K, Sameeullah M, Batool N, Lössl AG, Mariz F, Müller M, Mirza B, Waheed MT (2022). Inducible expression of human papillomavirus-16 L1 capsomeres in the plastomes of Nicotiana tabacum: Transplastomic plants develop normal flowers and pollen. Biotechnol. Appl. Biochem. 2022; 69 (2): 596–611. doi:10.1002/bab.2136 https://doi.org/10.1002/bab.2136
https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bab.2136Saba K, Sameeullah M, Asghar A, Gottschamel J, Latif S, Lössl AG, Mirza B, Mirza O, Waheed MT (2020). Expression of ESAT-6 antigen from Mycobacterium tuberculosis in broccoli: An edible plant. Biotechnol Appl Biochem. 67:148-157. doi: 10.1002/bab.1867.
https://doi.org/10.1002/bab.1867
https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bab.1867Saba K, Gottschamel J, Younus I, Syed T, Gull K, Lössl AG, Mirza B, Waheed MT (2019). Chloroplast-based inducible expression of ESAT-6 antigen for development of a plant-based vaccine against tuberculosis. J Biotechnol. 305:1-10. https://doi.org/10.1016/j.jbiotec.2019.08.016 https://www.sciencedirect.com/science/article/abs/pii/S016816561930834X?via%3Dihub
Gottschamel J and Lössl AG (2016): Chloroplast-Based Expression of Recombinant Proteins by Gateway Cloning Technology. Recombinant Proteins from Plants: Methods and Protocols, Methods Mol Biol. 1385: 3-27
https://doi.org/10.1007/978-1-4939-3289-4_1
https://link.springer.com/protocol/10.1007/978-1-4939-3289-4_1
Gottschamel J, Lössl AG, Ruf S, Wang YL, Skaugen M, Bock R, Clarke JL (2016): Production of dengue virus envelope protein domain III-based antigens in tobacco chloroplasts using inducible and constitutive expression systems. Plant Mol Biol. 91:497-512
https://doi.org/10.1007/s11103-016-0484-5
https://link.springer.com/article/10.1007/s11103-016-0484-5 OpenAccess
Waheed MT, Sameeullah M, Khan FA, Syed T, Ilahi M, Gottschamel J, and Lössl AG (2016): Need of cost-effective vaccines in developing countries: What plant biotechnology can offer? SpringerPlus 5: 65
https://doi.org/10.1186/s40064-016-1713-8 https://springerplus.springeropen.com/articles/10.1186/s40064-016-1713-8 OpenAccess
Ahmad N, Michoux F, Lössl AG, and Nixon PJ (2016): Challenges and perspectives in commercializing plastid transformation technology. J Exp Bot 67: 5945-5960
https://doi.org/10.1093/jxb/erw360
https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erw360 Open AccessWaheed MT, Ismail H, Gottschamel J, Mirza B, and Lössl AG (2015) Plastids: the Green Frontiers for Vaccine Production. Frontiers in Plant Science 6: 1005
https://doi.org/10.3389/fpls.2015.01005 Open Accesshttps://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2015.01005/full
Hassan SW, Waheed MT, Mueller, Clarke JL, Shinwari ZK and Lössl AG (2014) Expression of HPV-16 L1 capsomeres with glutathione-S-transferase as a fusion protein in tobacco plastids: an approach for a capsomere-based HPV vaccine. Human Vaccines & Immunotherapeutics 10: (10) 2975-2982 https://doi.org/10.4161/21645515.2014.970973
https://www.tandfonline.com/doi/full/10.4161/21645515.2014.970973 Open Access
Gottschamel J, Waheed MT, Clarke J, Lössl AG (2013): A novel Chloroplast transformation vector compatible with the Gateway recombination cloning technology, Transgenic Research 22: 1273-1278
https://doi.org/10.1007/s11248-013-9726-3
https://link.springer.com/article/10.1007/s11248-013-9726-3Clarke JL, Waheed MT, Lössl AG, I Martinussen, H Daniell (2013): Review. How can plant genetic engineering contribute to cost-effective fish vaccine development for promoting sustainable aquaculture? Plant Mol Biol 83: 33–34
https://doi.org/10.1007/s11103-013-0081-9
https://link.springer.com/article/10.1007/s11103-013-0081-9 Open AccessLössl AG, Clarke JL (2013): Conference Scene: Molecular pharming: manufacturing medicines in plants. Immunotherapy 5: 9-12 https://doi.org/10.2217/imt.12.146
https://www.tandfonline.com/doi/full/10.2217/imt.12.146 Open AccessWaheed MT, Gottschamel J, Hassan SW and Lössl A (2012): Plant-derived vaccines. An approach for affordable vaccines against cervical cancer. Human Vaccines 8: (3) 403-406
https://www.tandfonline.com/doi/full/10.4161/hv.18568 Open access
Lössl A and Waheed MT (2011) Chloroplast-derived vaccines against human diseases: achievements, challenges and scopes. Review. Plant Biotechnology J. 9: 527-539
https://doi.org/10.1111/j.1467-7652.2011.00615.x
https://onlinelibrary.wiley.com/doi/10.1111/j.1467-7652.2011.00615.x Open AccessWaheed MT, Thönes N, Müller M, Hassan SW, Gottschamel J, Lössl E, Kaul HP, Lössl A (2011) Plastid expression of a double-pentameric vaccine candidate containing human papillomavirus (HPV)-16 L1 antigen fused with LTB as adjuvant: transplastomic plants show pleiotropic phenotypes. Plant Biotechnology J. 9: 651-660
https://doi.org/10.1111/j.1467-7652.2011.00612.x
https://onlinelibrary.wiley.com/doi/10.1111/j.1467-7652.2011.00612.x Open AccessHassan SW, Waheed MT and Lössl A (2011) New areas of plant-made pharmaceuticals. Recombinant Pharmaceutical Manufacturing from Plants - The Future of Molecular Farming. Meeting Report. Expert Reviews of Vaccines 10: 151-153
https://doi.org/10.1586/erv.10.166
https://www.tandfonline.com/doi/full/10.1586/erv.10.166 Open Access
Waheed MT, Thönes N, Müller M, Hassan SW, Lössl E, Kaul HP, Lössl A (2010) Transplastomic expression of a modified human papillomavirus L1 protein leading to the assembly of capsomeres in tobacco: A step towards cost effective second generation vaccines. Transgenic Research 20: 271-282
https://doi.org/10.1007/s11248-010-9415-4
https://link.springer.com/article/10.1007/s11248-010-9415-4
Dr. Andreas Lössl, Professor für Agrarmanagement
andreas.loessl@iu.org
Andreas Lössls research group focusses on Plant Sciences, Biotechnology and Biopharming, mainly on plant organelles as bio factories. At the LMU in Munich he first managed to synthesize polyesters in chloroplasts transformed with a bacterial three-gene operon (Lössl et al. 2003). Further research focused on novel systems for secure production of plant-derived pharmaceuticals (Lössl et al. 2005). To further increase the security of plastid-made pharmaceuticals, they extended this system for the expression of various vaccine candidates.Prof. Dr. Mohammad Tahir Waheed, Dept of Biochemistry of Quaid-i-Azam University, Islamabad, Pakistan
Prof. Dr. Mohammad Tahir Waheed is working in Biotechnology and Plant Biopharming for more than two decades. He is Professor at the Dept of Biochemistry of Quaid-i-Azam University in Pakistan. As head of a Plant Biotech Laboratory he possesses excellent expertise and experience of project management and vaccine development using plants as biofactories. As a principal investigator he has extensive experience in vector design, gene expression and genetic engineering.